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KMID : 0364820140500020114
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Korean Journal of Microbiology
2014 Volume.50 No. 2 p.114 ~ p.118
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bla Genotype and Molecular Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Chungcheong Regional Hospitals
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Yook Keun-Dol
Yang Byoung-Seon
Park Jin-Sook
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Abstract
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A total of 122 ESBL-producing intestinal bacteria were collected from regional hospitals in the Chungcheong area.
Combination disk test (CDT) was performed for antimaicrobial susceptability using cefotaxime and cefotaxime/
clavulanate according to Clinical Laboratory Standard Institute (CLSI). Mutiplex PCR using specific primers was
performed for a detection of ESBL-genotypes and enterobacterial repetitive intergenic consensus (ERIC)-PCR was
carried out for the tracking of molecular epidemiology. In the confirmation test using CDT, 73 out of 76 (96.1%)
ESBL-producing Escherichia coli and 43 out of 46 (93.4%) ESBL-producing Klebsiella pnemoniae were positive. In
the multiplex PCR, 60.5% of E. coil were positive for CTX-M-2 type gene and 56.5% of K. pneumoniae were
positive for VEB -1 type gene. In the ERIC-PCR, E. coil isolates formed 5 clusters and K. pneumoniae isolates were
grouped into 4 clusters depending on region. Genotypes of clinical isolates are useful for detection and
differentiation of ESBL producing intestinal bacteria. The ERIC-PCR method is thought to be helpful for establishing
a regional surveillance system for infection due to its formation of different clusters depending on region.
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KEYWORD
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Escherichia coli, Klebsiella pnemoniae, CDT, ERIC-PCR, ESBL, multiplex PCR
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